Engineering Escherichia coli cells for cellobiose assimilation through a phosphorolytic mechanism.
نویسندگان
چکیده
We report here the first engineering effort for Escherichia coli biocatalysts to assimilate cellobiose through a phosphorolytic mechanism. Cytoplasmic expression of the Saccharophagus cellobiose phosphorylase was shown to enable E. coli to use cellobiose. Subsequent knockout and complementation studies provided solid evidence that the endogenous LacY was responsible for the transport of cellobiose.
منابع مشابه
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 78 5 شماره
صفحات -
تاریخ انتشار 2012